LOCUS       40924_23889       12069 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB765 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12069)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Gateway Binary Vectors with Organelle-Targeted Fluorescent Proteins 
            for Highly Sensitive Reporter Assay in Gene Expression Analysis of 
            Plants
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12069)
  AUTHORS   Momtaz S, Dutta A, Tanaka Y, Aboulela M, Nishimura K, Sugiura S, 
            Niwa T, Maeo K, Goto-Yamada S, Kimura T, Ishiguro S, Mano S, 
            Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane 
            University, Interdisciplinary Center for Science Research; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12069)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12069)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-OCT-2018) Contact:Tsuyoshi Nakagawa Shimane University, 
            Interdisciplinary Center for Science Research; 1060 Nishikawatsu, 
            Matsue, Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12069
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2071..2091
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2092..2808
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     terminator      2833..3085
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3119..3135)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3143..3159
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3167..3197)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3212..3233)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3269..3521)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3559..4854)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BBG75662.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(3559..4854)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(4906..5085)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5591..5615)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6136..6924
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7173..7761
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7947..8087)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8431..8625)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8694..9764)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10196..10822)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcatggct ccaaagaaga agagaaaggt catggtgagc
     2101 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2161 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2221 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2281 accttcacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac
     2341 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2401 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2461 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2521 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2581 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2641 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     2701 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     2761 ttcgtgaccg ccgccgggat cactcacggc atggacgagc tgtacaagta agcttagagc
     2821 tcgaatttcc ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt
     2881 gccggtcttg cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt
     2941 aacatgtaat gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta
     3001 tacatttaat acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc
     3061 gcggtgtcat ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatggt
     3121 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     3181 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaggctgaa
     3241 ttaggcgcgc ctatttctat taattcccga tctagtaaca tagatgacac cgcgcgcgat
     3301 aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc
     3361 gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt
     3421 acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga
     3481 ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggaaatt cgcctcgagt
     3541 gacttctctg attgcagact atttatacca accagcaaga aagtgtcgaa gtatgaagca
     3601 gaaggcgcag gctagagcct ggaaaacgag aagatggatg caaagagact tttctgattt
     3661 aggaccaaac aacctcaagt aaacattgac gagtgtccca tcttttgtgc cggtcagtaa
     3721 tccggtagca ggatcatacc ttgggagacg atggcgcgga catttcacaa tcccagcaag
     3781 ctgaggaagc gataatagaa ggttcagaac ttgaggaaga aagaagatga ggagggtttc
     3841 actgaagtga cccagaatac caacaactgc catagtcatt ccggcgaaga ctgtgtaagt
     3901 gtctccaaca aaaactgaag aaggatacca attgtaagca agcattgcca aggatgtggc
     3961 catcagcggt tgagtaagaa atatcgagaa ggcatgagct tggtgatact cgggatcaac
     4021 agatgctccg atttgcataa cgttgtgtat caggatagca gcggcaataa caactgtttg
     4081 accgatttca aggccattca gaccagcgtg gatgttaata gagtttgtac aaaagacagc
     4141 aagtagcccc atatataact tatatatcct tcctagatta agcacttcca agccaatata
     4201 agcaacaaga ggttttggta taacaatagt tgtatgacca gcataagcca tcaaaagtgg
     4261 aagggtagca aaagatggca ggacaagttt tactctccaa ggcacatcaa ggacatcatc
     4321 tacaaatcca agcaaaatca tgaagcaaat agatgctagt gctgcattat actccacaag
     4381 ccaattagaa tcttcagtga agttaaaata ctgaaatatt atcgccacga tcaaaaagac
     4441 aataccaaca acaataccca atgactctgg cactttaata tctccttgag gcgtgcctct
     4501 tttgttgata tcaaaaccaa acatgttacg tctcagaacg tatctggcag taacaggaat
     4561 cattttgaga gtgacgaaaa acccaaccaa gctaagcccc gcgttaatta gaatcgatcg
     4621 cttgagttca ttatcaacct tgtagtgaaa acagagaagg tatagataga gcgaacagag
     4681 aagagtagag atcacgaaga tcactcctaa tttcggcggc gcgagacgaa attcctcacc
     4741 ggaagcagaa accgtctttc gggtcatctt ctgctccgat ggcgctgagt tgggttccga
     4801 cgggtccggt ttgttgggaa ttgagatcga tgaagctctc ttacgagctg ccatatctta
     4861 ttgccccccg ggatcagatc tggattgaga gtgaatatga gactctaatt ggataccgag
     4921 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     4981 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     5041 actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     5101 taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc
     5161 tcatgatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     5221 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     5281 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     5341 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     5401 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     5461 ccctgagtgc ttgcggcagc gtgaagctga tccgtcgaga ttaatagaaa taaattcagc
     5521 ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     5581 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     5641 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     5701 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     5761 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     5821 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa
     5881 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     5941 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     6001 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     6061 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     6121 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     6181 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     6241 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     6301 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     6361 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     6421 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     6481 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     6541 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     6601 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     6661 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     6721 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     6781 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     6841 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     6901 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     6961 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     7021 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     7081 ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     7141 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     7201 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     7261 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     7321 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     7381 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     7441 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     7501 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     7561 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7621 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7681 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7741 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7801 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7861 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7921 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     7981 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     8041 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     8101 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     8161 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     8221 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
     8281 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
     8341 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
     8401 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
     8461 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
     8521 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
     8581 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
     8641 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
     8701 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
     8761 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
     8821 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
     8881 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
     8941 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
     9001 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
     9061 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
     9121 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
     9181 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
     9241 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
     9301 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
     9361 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
     9421 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
     9481 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
     9541 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
     9601 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
     9661 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
     9721 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
     9781 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
     9841 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
     9901 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
     9961 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
    10021 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
    10081 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
    10141 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
    10201 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
    10261 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
    10321 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
    10381 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
    10441 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
    10501 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
    10561 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    10621 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    10681 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    10741 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    10801 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    10861 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    10921 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    10981 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    11041 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    11101 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    11161 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    11221 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    11281 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    11341 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    11401 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    11461 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    11521 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    11581 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    11641 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    11701 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    11761 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    11821 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    11881 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    11941 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    12001 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
    12061 gataaacct
//
